Volumetric pipette, 1 mark, Class AS. The diffused brown graduations ensure excellent readability and high durability.
Made of borosilicate glass 3.3 and supplied with a batch certificate, compliant with DIN ISO 648.
Colour coding: compliant with ISO 1769 standard (e.g. Blue = 1 mL / 25 mL, Orange = 2 mL, White = 5 mL, Red = 10 mL / 50 mL, Yellow = 20 mL / 100 mL)
What is a single-mark volumetric pipette, class AS, used for?
The VP.J1S1
single-mark volumetric pipette delivers a single precise volume (1 mL) with
accuracy meeting class AS, the most demanding standard for volumetric
glassware. Unlike graduated pipettes that allow multiple measurements, it is
calibrated to deliver one fixed volume, making it ideal for precise dilutions,
standard preparation, titrations, and any analytical application where the
accuracy of the delivered volume is critical. The fast-drain design specific to
class AS ensures reliable, reproducible liquid delivery.
What does accuracy class AS mean and why does it matter?
Class AS
(ISO 648 standard) designates the highest level of volumetric accuracy for
glass pipettes, with tighter tolerances than class B. In practice, this means
minimal measurement uncertainty and excellent reproducibility across multiple
deliveries. This class is required in analytical, quality control, and research
laboratories where any volume deviation could affect the reliability of an
analytical result, titration, or standard preparation.
Why fused brown graduations instead of standard markings?
Fused brown
graduations offer optimal contrast against most laboratory liquids, including
colored or slightly opaque solutions, where standard white markings would be
less readable. The fusion technique (enamel fired into the glass) also ensures
excellent resistance to abrasion, solvents, and repeated washing cycles, unlike
simple printing, which could fade over time. This combination of readability
and durability is especially valued in intensive use.
What does the batch certificate supplied with this pipette provide?
The batch
certificate confirms that the pipette was manufactured and tested in compliance
with the DIN ISO 648 standard, with production batch traceability. This
document is essential for laboratories operating under accreditation (ISO
17025), in regulated environments (pharmaceutical, food industry), or requiring
quality documentation for internal or external audits. It allows the
metrological compliance of the instrument used in a protocol or analysis to be
justified, strengthening the credibility of the results produced.
Why choose borosilicate glass 3.3 for this pipette?
Borosilicate
glass 3.3 offers excellent chemical resistance to acids, bases, and organic
solvents commonly used in laboratories, along with good thermal and mechanical
stability. This robustness ensures the pipette's dimensional stability over
time, preserving class AS accuracy even after many use and cleaning cycles. It
is the reference material for high-precision volumetric glassware intended for
repeated professional use.
How do I use this pipette correctly to ensure the stated accuracy?
To preserve
class AS accuracy: hold the pipette vertically when drawing up liquid and
reading the level (at the bottom of the meniscus). Fill to the calibration
mark, avoiding air bubbles. For draining (class AS, fast-drain), let the liquid
flow freely along the wall without blowing out the last drop, unless the
protocol specifies otherwise. Clean immediately after use to prevent residues
that could affect the reading or contaminate the next use.
Is this pipette autoclavable and how do I sterilize it if needed?
Yes, the
VP.J1S1 pipette is autoclavable, allowing sterilization by moist heat
(typically 121 °C, 15–20 minutes) without risk to the borosilicate glass
integrity or class AS accuracy. Note, however, that the product is supplied
non-sterile as standard; sterilization should be performed locally if the
protocol requires it (biology, microbiology). It is recommended to let the
pipette cool before immediate reuse to avoid thermal shock.
In which application contexts is this pipette recommended?
This 1 mL
class AS volumetric pipette is particularly suited to analytical chemistry
laboratories (standard preparation, precise dilutions), pharmaceutical or food
quality control laboratories requiring traceability documentation, volumetric
titrations demanding high accuracy, and academic or industrial research where
reproducibility of delivered volumes determines the validity of results. The 1
mL format is especially suited to small sample volumes or costly reagents.
Does the color code of this pipette comply with a standard?
Yes. The color codes used per capacity (blue for 1 mL, orange for 2 mL, white for 5 mL, red for 10 mL, yellow for 20 mL, blue for 25 mL, red for 50 mL, yellow for 100 mL) comply with the ISO 1769 standard for color coding of laboratory pipettes. This system allows quick, unambiguous identification of nominal capacity, reducing handling errors, especially when several pipettes of different capacities are used simultaneously at the same workstation.